237 Screening of soluble metabolites produced by probiotics on human dermal fibroblasts for anti-aging properties

Dermal fibroblasts are producing the connective tissue with extracellular matrix (ECM) components, where type I collagen is major structural protein. During aging production ECM proteins decreases and synthesis of degrading metalloproteinases (MMPs) increases, leading to imbalanced homeostasis wrinkle formation. Postbiotics, metabolites produced by bacteria, incubated on a monolayer human dermal were used model in vitro effects oral probiotic supplementation dermis through so-called gut-skin-axis. Eight strain postbiotics (Bifidobacterium animalis ssp. lactis strains Bi-07, Bl-04 B420; Lactiplantibacillus plantarum Lp-12407 Lp-12418; Lacticaseibacillus paracasei Lpc-37; rhamnosus HN001 Ligilactobacillus salivarius Ls-33) devoid bacterial cells screened. The applied for 72h, without or tumor necrosis factor alpha (TNF-α) induce MMPs. anti-aging properties evaluated measuring fibroblast cell viability pro-collagen, MMP-1/type pro-collagen ratio, TNF-α interleukin 6 (IL-6). challenge induced increase fibroblast-produced TNF-α, as well MMP-1 IL-6, expected. Clear species dependent differences noticed. All postbiotics, but especially Bifidobacterium strains, promoted from B420 maintained at high level even under stress. bifidobacterial also preserved MMP-1/pro-collagen ratio closest transforming growth beta 1 (TGF-β1) level, positive control production. results indicate that B. Bl-04, could support metabolism skin.